MOUSE ANTI BOVINE INTERLEUKIN-4

Published customer image:
Mouse anti Bovine Interleukin-4 antibody, clone CC313 (MCA2371) used as a capture reagent for the detection of interleukin-4 in supernatants of cultured bovine cells using a sandwich ELISA together with biotinylated clone CC314 as a detection reagent.
Image caption:
CD1b+ L-DCs are efficient APC for the priming of allogeneic naive T cells. Sorted CD1b+ L-DCs were incubated at different ratios with 10⁵ allogeneic CD4+ T cells (A) or CD62L+ T cells (C), and tested for proliferation by [³H]-thymidine incorporation after six days of culture. Data express the median (quartiles) of counts per minute (cpm) from triplicates for each condition. After three days of culture, supernatants were sampled and IFN-γ (black bars) and IL-4 (grey bars) production analysed by ELISA (B, D). The results shown are from one representative experiment out of four performed on different sheep (A) or out of two performed on one sheep(C).

From: Olivier M, Foret B, Le Vern Y, Guilloteau LA (2012)
Capacities of Migrating CD1b+ Lymph Dendritic Cells to Present Salmonella Antigens to Naive T Cells.
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This image is from an open access article distributed under terms of a.

Published customer image:
Mouse anti Bovine Interleukin-4 antibody, clone CC313 (MCA2371) used as a capture reagent for the detection of interleukin-4 in supernatants of cultured bovine cells using a sandwich ELISA together with biotinylated clone CC314 (MCA2372B) as a detection reagent.
Image caption:
CD1b+ L-DCs are efficient APC for the priming of allogeneic naive T cells. Sorted CD1b+ L-DCs were incubated at different ratios with 10⁵ allogeneic CD4+ T cells (A) or CD62L+ T cells (C), and tested for proliferation by [³H]-thymidine incorporation after six days of culture. Data express the median (quartiles) of counts per minute (cpm) from triplicates for each condition. After three days of culture, supernatants were sampled and IFN-γ (black bars) and IL-4 (grey bars) production analysed by ELISA (B, D). The results shown are from one representative experiment out of four performed on different sheep (A) or out of two performed on one sheep(C).

From: Olivier M, Foret B, Le Vern Y, Guilloteau LA (2012)
Capacities of Migrating CD1b+ Lymph Dendritic Cells to Present Salmonella Antigens to Naive T Cells.
PLoS ONE 7(1): e30430.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.